Composite
Part:BBa_K2924036:Design
Designed by: Andreas Nakielski Group: iGEM19_Duesseldorf (2019-10-11)
Pcpc560 + RBS + mVenus + Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 565
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1191
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 589
Illegal AgeI site found at 712 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed to test the strength of the promoter Pcpc560 with a reporter gene
Source
The plasmid, the reporter gene and the promoter were provided by Anna Behle1
References
1: Behle, A., Saake P., Axmann, I. M. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.