Composite

Part:BBa_K2924036:Design

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-11)


Pcpc560 + RBS + mVenus + Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 589
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to test the strength of the promoter Pcpc560 with a reporter gene

Source

The plasmid, the reporter gene and the promoter were provided by Anna Behle1

References

1: Behle, A., Saake P., Axmann, I. M. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.